![]() The software also designs primers for flanking exons and introns in a gene, though there is no mention of it on the official Primer Premier page on the Premier Biosoft website. It is also understood that the primers designed by Primer Premier are effective and confirm to the design guidelines laid for designing primers for housekeeping genes with a high G+C%. The software has a built-in nucleotide-protein and protein-nucleotide sequence translator which can be used for designing degenerate primers for codons that code for multiple amino acid sequences. The Primer Premier software supports motif analysis and ORF search along with finding restriction cut sites on the nucleotide sequence. The software also designs tagged and nested primers for multiplex PCR reactions. The software supports design of degenerate primers on alignments for amplifying a related set of nucleotide sequences for detecting many common traits amongst organisms and to determine heredity. Primer Premier software combines design of primers for various PCR applications under one common platform.
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